Nature

TaqMan RT-PCR assay coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type

Following real-time RT-PCR, each amplification product was diluted 10-fold with water and 0.5 μl the diluted products were then subjected to capillary electrophoresis in an ABI Prism 3100 Genetic ...
Nature

Real-Time t(14;18)(q32;q21) PCR Assay Combined with High-Resolution Capillary Electrophoresis: A Novel and Rapid Approach that Allows Accurate Quantitation and Size ...

Real-time polymerase chain reaction (PCR) for detection and quantitation of bcl-2/JH fusion sequences characteristic of t(14;18) (q32;q21) is being used increasingly to monitor tumor burden and the ...
The Scientist

Optimizing PCR: Proven Tips and Troubleshooting Tricks

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...
JSTOR Daily

Improvements of Polymerase Chain Reaction and Capillary Electrophoresis Single-Strand Conformation Polymorphism Methods in Microbial Ecology: Toward a High-Throughput Method ...

The molecular signature of bacteria from soil ecosystems is an important tool for studying microbial ecology and biogeography. However, a high-throughput technology is needed for such studies. In this ...
JSTOR Daily

Evaluation of the Bacterial Vaginal Flora of 20 Postmenopausal Women by Direct (Nugent Score) and Molecular (Polymerase Chain Reaction and Denaturing Gradient Gel ...

To monitor the bacterial vaginal microflora of postmenopausal women, several approaches were used: (1) Nugent scoring of Gram-stained smears from vaginal swabs; (2) testing for bacteria, using ...