Real-time PCR, or quantitative qPCR, is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle. For quantitative reverse transcription PCR ...

Detection of PCR products in real time can be accomplished by using fluorescent dyes or probes. Fluorescently labeled probes detect the amount of specific double-stranded DNA sequences. While ...

But what is real time RT–PCR? How does it work? How is it different from PCR? And what does this have to do with nuclear technology? Here’s a handy overview of the technique, how it works and a few ...

GenEx System Aids in the Selection of Reference Genes for Standardizing mRNA Measurements Quantitative real-time PCR (qPCR) is currently the prime technique to measure gene expression. When properly ...

The accuracy of the RT-PCR test depends on timing; tests are best given within 10 days of COVID-19 symptom development. As the COVID-19 pandemic continues to spread across the country, many nurse ...

Respiratory syncytial virus (RSV) was detected easiest when using reverse transcription polymerase chain reaction (RT-PCR) tests in a pediatric population. Reverse transcription polymerase chain ...

In a recent study published in Lancet Microbe, researchers compare the potential of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) genotyping assays with whole-genome sequencing ...

One of the best ways to figure out how far a virus like COVID-19 has spread in a community is to test for it. This step-by-step guide explains how coronavirus swab-the-nose-and-throat tests work, and ...

During the early stages of the coronavirus disease 2019 (COVID-19), rapid testing was imperative for public health policymakers and health care workers in order to determine where and what measures ...