What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM ...

Three-dimensional structured illumination microscopy (3D-SIM) provides excellent optical sectioning and doubles the resolution in all dimensions compared with wide-field microscopy. However, its much ...

We developed 4Pi-SIM, which integrates 4Pi microscopy with structured illumination microscopy to achieve isotropic optical resolution through interference in both illumination and detection. We ...

When single-molecule super-resolution microscopes were first commercialized some 15 years ago, they made headlines for their ability to resolve individual molecules and structures at the nanometer ...

Open-source core technology embedded inside projector hardware enables high-speed, auto-polarization modulated 3D structured illumination microscopy (SIM) imaging. 3DSIM reconstruction of plant and ...

Today's super-resolution microscopes have made it possible to observe the nanoscale world with unprecedented detail. However, they require fluorescent tags, which reveal structural details but provide ...

Peking University, Aug 18, 2025: Professor Xi Peng’s team from the College of Future Technology at Peking University has recently developed a triangle structured illumination microscopy (SIM), which ...