Binding buffer: 5 mM Tris-HCl (pH 7.5), 0.5 mM EDTA, 1 M NaCl Breaking buffer: 10 mM Tris-HCl (pH 7.5), 1% Triton-X 100, 1% SDS, 100 mM NaCl, 1 mM EDTA Primers 1 and 2 are used for preamplification of ...
The design of short LAM-PCR amplicons that do not exceed several hundred base pairs is critical for the efficiency of each analysis. Amplicons should be long enough to be resolved by electropheresis ...
Background Lupus nephritis (LN) is the most serious complication of SLE. Interstitial fibrosis is the dominant pathological ...
2016: Jean-Pierre Sauvage, Sir J. Fraser Stoddart and Bernard L. Feringa were jointly awarded the Nobel Prize in Chemistry "for the design and synthesis of molecular machines." The trio took chemistry ...
The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade ...
The Therapeutic Goods Administration (TGA) says 21 sunscreens that share the same base formulation have been recalled, paused or are under its review. The TGA says it has "significant concerns" about ...
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Your doctor may order a creatinine test to assess the health of your kidneys. A high creatinine level could be a sign of an issue such as chronic kidney disease (CKD). Creatinine is a waste product ...
Abstract: The amount of DNA strands available in a biological sample is a major limitation for many genomic bioanalyses. To amplify the traces of DNA strands, polymerase chain reaction (PCR) is widely ...